Scroll down to watch the video of McBusted's cycle race.
The six boys, who will kick off their supergroup tour in April, donned jodhpurs, flares and shellsuit jackets to hold their own cycle race to promote the campaign.
James Bourne and Matt Willis from Busted, and Tom Fletcher, Dougie Poynter, Harry Judd and Danny Jones from McFly, star in a video clip which shows the six singers compete in a short race.
"Hi, we're McBusted and we want you to sign up to the Sainsbury's Sport Relief games," the group shout in unison at the start of the clip, followed by whoops, cheers and the ringing of bells.
Riding different bicycles ranging from the 1800s to the 1990s, each singer is dressed according to their era.
Tom, who represented the seventies in snug flares and a garish mustard yellow shirt, looked in high spirits as he was presented with a Chopper.
"I'm the sexy era," said Tom as he wiggled his hips and busted out his best disco moves.
Representing the eighties, fellow bandmate James rocked a shellsuit jacket and comic printed t-shirt. "Who wouldn't want to cycle around in fancy dress?" James is filmed asking the camera.
Despite being given the oldest bicycle in the group, the penny farthing, Matt was crowned the winner, screaming "Yes" as he was helped off the 1800s bicycle.
The group, who entertained with their stretches, jumps and matching pink t-shirts and headbands, encouraged people to get involved in the charity challenge by cycling, running or swimming.
"We've been involved with Sport Relief for the past few years, running a Mile in 2006 and visiting projects in Uganda a year later, to see how money raised by ordinary people for Comic Relief is spent changing people's lives," said Tom.
"It was an emotional but inspiring trip and we want everyone to show us their awesome pedal power by signing up to help raise cash this year."
Money raised from Sport Relief will be spent helping some of the poorest and most disadvantaged people in the UK and around the world. Sainsbury's Sport Relief Games will take place from 21 to 23 March.