And just like last year, it was her young grandchildren who stole the show, causing mischief as they clambered over the Royal Palace balcony.
CLICK ABOVE FOR GALLERY
The balcony at Copenhagen's Marselisbog Palace was transformed into a stage for the youngsters, who were watched by the thrilled onlookers who had gathered to celebrate their Queen’s birthday.
Missing from the photographs this year however was little Athena, whose parents are Prince Joachim and his French-born wife Princess Marie.
Her step-brothers, Nikolai and Felix posed this year with their parents while her elder brother Henrik, who wrapped up warm in a red V-neck jumper, was helped by his mother Princess Marie.
The family scene was completed by Joachim's brother Crown Prince Frederik and his wife Princess Mary.
Their two eldest children Christian and Isabella smiled out at the crowds, while three-year-old twins Josephine and Vincent, leaned on the balcony walls.
At one point Vincent attempted to climb over the walls and was watched by his laughing parents.
Margrethe's birthday on 16 April always unites the Danish royal family with the Danish public who turn out in their thousands to celebrate the date.
Queen Margrethe was joined by her husband, Prince Henri.
Margrethe is very close to Britain's Queen Elizabeth II and speaking in 2012 she revealed how she had always "admired" her and that she has helped influence herself as a Queen after watching how dedicated Elizabeth is to her nation.
Click here to 10 facts about Denmark’s Queen