The pop sensation played beer pong with the show host, and sang a few lines from Taylor's smash hit song, showing her fun sense of humour.
Fans were quick to praise the 34-year-old on the show, with one writing: "Awww I just love @britneyspears she is sooo cute on Jonathan Ross!"
"Definitely a fan of @britneyspears again after seeing her on Jonathan Ross show," another added.
The Oops, I Did It Again singer shares two sons. 11-year-old Sean and ten-year-old Jayden, with ex-husband Kevin Federline, and recently opened up about how she worries about them becoming teenagers.
Britney joined Jonathan and played a game of beer pong
Speaking on ITV's Lorraine, she said: "It's a little strange. I'm nervous because I know 12 and 13 and 14 and 15 are going to be really scary. I'm going to be like, 'Mum please come to the house and help me out!' But no, it's a great age. They are still babies."
She also opened up about being single again after her recent split film and TV producer Charlie Ebersol, saying: "I am in a really good place right now. I am loving and learning about myself every day and learning who I am.
The popstar tried helium before singing Taylor Swift tune Shake It Off
"It's awakening, it's cool. It’s okay to be single — it totally is."
The star recently shared an hilarious video of her sons pulling a prank on her by jumping out at her, causing her to scream and fall back, shouting Sean's middle name in anger. The star clearly saw the funny side of their antics eventually, and cheekily chastised the pair in a loving Instagram post.