The Queen has two runners on the second day - Pied Piper in The Queen's Vase and Spring is Sprung in The Windsor Castle Stakes.
Royal Ascot is the perfect excuse for racegoers to wear their fanciest frocks and biggest hats. Take a look at all the highlights from day two...
WATCH: Charles and Camilla join Sophie Wessex and Princess Anne for day two of Royal Ascot
The Earl and the Countess of Wessex made quite the stylish entrance for day two of the races.
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Sophie wowed in an A-line floral skirt by Suzannah, with an ARossGirl x Soler white blouse with court shoes and a feathered lavender Jane Taylor hat.
Prince Charles and Camilla were pictured arriving at the racecouse by car.
Camilla looked elegant in a champagne chiffon dress by Fiona Clare and a wide-brimmed Philip Treacy hat.
The Princess Royal stepped out for day two of Royal Ascot wearing a chic green jacquard midi dress with cream accessories.
Prince Edward donned a nautical-themed face mask to speak to other guests on the racecourse.
The Queen's nephew, David Armstrong-Jones, 2nd Earl of Snowdon, made an appearance at the races. He has previously accompanied his aunt at Royal Ascot, riding in the carriage procession with Her Majesty in 2018.
The Duchess of Cornwall's first husband, Andrew Parker Bowles, was among the guests on day two of the races.
The pair share two children, Tom Parker Bowles and Laura Lopes.
Good Morning Britain presenter Charlotte Hawkins looked lovely in a white shirt dress with floral detailing on its collar and belt.
ITV racing presenter Francesca Cumani wore a black and cream tiered dress with a hat by Rachel Trevor Morgan.