Showing off her super slender figure in a revealing black thong and a sheer bodysuit from the La Perla 'Cage' collection for the performance, the pop star performed a raunchy rendition of her hit alongside her husband Jay Z.
The usually private power couple enjoyed some intimate back-to-back slow dancing and Jay Z, 44, even grabbed his wife's famous bottom and gave her a kiss mid-song before the 16,000-strong audience.
Beyoncé, 32, who last month completed a 22-day vegan diet challenge with her rapper husband, debuted her flat midriff and slender legs in the sheer ensemble with a glittering bra and crystal-embellished fishnets.
The mum-of-one was sexy with her short locks in a wet hair-do, along with a smokey-eye effect, as she completed a sexy dance routine on a rotating chair.
This isn't the first time fans were treated to a joint performance from the parents of two-year-old Blue Ivy.
Jay Z makes an appearance on Beyoncé's new single Drunk In Love, and the superstar sang on her rapper hubby’s Grammy-nominated song Part II (On The Run).
The 16-time Grammy winner is up for eight other Grammys, including Best Rap Album for Magna Carta... Holy Grail.
Jay Z took home an early award during the ceremony, scooping the prize for Best Rap/Sung Collaboration for Holy Grail, featuring Justin Timberlake.