The Dancing with the Stars professional turned heads in a blue lace crop top and tiny denim hot pants as she posed on stage with the country superstar – who celebrated her 75th birthday.
The photo is from Dolly's Netflix show, Heartstrings, which saw Julianne play Jolene in the debut episode.
WATCH: Julianne Hough shows off her physique and her dance moves insider her LA home
Julianne sweetly captioned the photo: "Happy Birthday to the Queen Butterfly @dollyparton. "Thank you for leading by example. Thank you for being an inspiration. And thank you for teaching us to dream big! I will always love you."
Fans loved the trip down memory lane – and Julianne's toned dancer's physique – with many rushing to comment on the snap.
"@juleshough I didn’t think anybody could take my attention away from Dolly when she is on stage. I was clearly wrong. Congratulations! gushed one.
Julianne stunned fans in her crop top and tiny shorts
"You look perfect," said another. A third added: "Literally my two favourite people! That episode was sooo good!" A fourth wrote: "What a goddess."
Julianne was in a jovial mood on Tuesday and earlier danced up a storm inside her LA home, displaying her flawless physique.
The 32-year-old showed off her impressive moves - see the video above - in a skintight exercise outfit complete with green leggings and a crop top.
Dancing in the living room of her house in the Hollywood Hills, Julianne had clearly woken up in a bright and bubbly mood.
Julianne makes exercise a priority
After swishing her hair and her hips around the star sent another video message to her fans and said: "I just want to scream. Honestly, sometimes you just need to scream. I feel so good today and I just need to get that energy out."
Julianne maintains her toned figure by being active and listening to what her body needs.
She recently opened up to People magazine to share some of her secrets and said the main thing is "just try to move your body every day".
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