He's been her manager since the Nineties when she made her teenage debut in the multi-platinum-selling group Destiny's Child.
But is has been confirmed that Beyonce and her father Matthew Knowles will no longer be working together "on a business level".
Beyonce has said: "I am grateful for everything he has taught me. I grew up watching both he and my mother manage and own their businesses."
The 29-year-old superstar added: "They were hard-working entrepreneurs and I will continue to follow in their footsteps."
No reason has been given for what led to the split but Matthew has said the decision was mutual.
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