However, on Friday, Ryan departed from the popular ABC show after seven years, and Ali was one of the first to mark the bittersweet occasion with a sweet tribute on social media.
Taking to Instagram, the TV star shared a photo of her and Ryan linking arms in the ABC studio, alongside the message: "Had the best time with you @ryanseacrest."
Fans were quick to comment on the post, with one writing: "I hope you still get to co-host, you two were so much fun," while another wrote: "You were a great duo when you filled in for Kelly." A third added: "You guys were awesome together. You need a show to bring your smiles."
Ryan will not only be missed by Ali, but by his co-host of seven years, Kelly. During a speech in the middle of Friday's emotional episode, Kelly admitted that their relationship was "complicated," and the reason made Ryan, 48, laugh.
Kelly, 52, said through tears: "Although we have been friends for decades and decades, I feel I have gained a younger brother, slash older son."
The audience and Ryan giggled. "It's very complicated," she then confessed. "I'm so grateful that our success together on this show has led to us developing an even closer but co-dependent relationship."
Kelly's husband, Mark Consuelos will now be taking over Ryan's hosting duties and he appeared at the end of the show with a cake for the American Idol alum.
The husband and wife duo recently dropped the first teaser for Live with Kelly and Mark. Ryan announced he was leaving the ABC show in February.
The star joined the show back in 2017, following in the footsteps of Michael Strahan and before him Regis Philbin.
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