The Queen's granddaughter was aptly dressed in a white shirt dress from Ralph Lauren and navy-coloured wedge shoes which were teamed with a black leather crossbody and gold earrings.
WATCH: The royal family at Wimbledon over the years
Not to be outdone in the style stakes, her rugby star husband Mike put on a dapper display in a cream jacket and dark trousers combination, teamed with grey suede shoes and a stripy tie.
The two tennis stars have both overcome injuries to make it to the All England Club in a bid to land another grand slam.
Mike and Zara Tindall on day two of Wimbledon
Serena, 40, is making her comeback after limping out of last year's first-round match in tears due to a leg injury. She will play France's Harmony Tan, while 36-year-old Rafael faces Argentine tennis player Francisco Cerundolo.
GET THE LOOK: Linen shirt dress, £265, Ralph Lauren
The matches follow on from the opening day of the tournament which saw 19-year-old Emma Raducanu make her winning Centre Court debut and Andy Murray triumphing to the next round.
Cliff Richard made another Wimbledon appearance
Sir Cliff Richard made another appearance at Wimbledon this week, he looked slick in a teal blue suit and gold-framed sunglasses.
Bear Grylls and his wife Shara looked lovely as they made an appearance together.
Andrew Ridgeley and his guest happily stopped for photos.
Zoe Wanamaker and her husband Gawn Grainger were spotted in the crowd.
Joe Sugg was among the stars at the Evian VIP Suite, certified as carbon neutral by The Carbon Trust, at Wimbledon.
Andy, 35, smiled at fans as he walked through the grounds for practice ahead of his second round with American tennis player John Isner, on Wednesday.
Meanwhile, other celebrity attendees included AJ Tracey, Hana Cross, Tom Grennan, Saffron Barker and Ovie Soko.
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